姓名: 施能朗 Neng-Lang Shih
職稱:

助理教授兼任研發處技術推廣及服務組組長

學歷:

國立中興大學 農學院 獸醫學學士
國立台灣大學 農學院 獸醫學碩士
國立台灣大學 醫學院 毒理學博士

經歷:

台北榮民總醫院 醫學研究部 研究助理
中央研究院生物醫學研究所 研究助理
中央研究院生物醫學研究所 博士後研究
國立高雄大學 生命科學系 助理教授

辦公室: 理學院大樓 609 室
實驗室:

心臟血管生物實驗室(理學院大樓 609-1 室)

聯絡電話:

886-7-5919448,校內分機 7448

E-Mail:

shihnl@nuk.edu.tw

學術專長:

心臟學
毒理學
動物疾病模式
獸醫學
分子生物學

開授課程:

(大學部) 比較解剖學及實驗普通生物學實驗寄生蟲學胚胎學
(研究所) 免疫學
(通識課程) 身體防禦系統

研究計劃:

1.計畫名稱:日本鰻魚成熟過程中血管生成之調控與應用
計畫單位:國科會
計畫編號: NSC 96-2313-B-390-002-MY3
執行期間:96.08.01~99.07.31
參與工作:共同主持人

 



期刊論文:

1. Wang, D.L.; Chen, J.J.; Shih, N.L.; Kao, Y.C.; Hsu, K.H.; Huang, W.Y.; Liew, C.C.
(1992) “Endothelin stimulates cardiac α- and β-myosin heavy chain gene expression.”
Biochem. Biophys. Res. Commun. 183:1260-1265.

 

2. Chen, J.J.; Hsu, K.H.; Kao, Y.C.; Shih, N.L.; Lien, W.P.
(1992) “Developmental regulation of myosin heavy chain gene expression in cultured rat cardiac muscle cells.” Acta Cardiol. Sinica 8:12-21.

 

3. Chen, J.J.; Wang, D.L.; Shih, N.L.; Hsu, K.H.; Lien, W.P.; Liew, C.C.
(1992) ”Regulation of human cardiac myosin heavy chain genes : the effect of catecholamine.” Biochem. Biophys. Res. Commun. 188:547-553.

 

4. Chen, J.J.; Shih, N.L.; Hsu, K.H.; Liew, C.C.
(1993) “Serial deletion constructs of human cardiac myosin heavy chain genes generated by PCR amplification. “ Mol. Cell. Biochem. 124:81-84.

 

5. Shyu, K.G.; Chen, J.J.; Shih, N.L.; Wang, D.L.; Chang, H.; Lien, W.P.; Liew, C.C.
(1995) “Regulation of human cardiac myosin heavy chain genes by cyclic mechanical stretch in cultured cardiocytes.” Biochem. Biophys. Res. Commun. 210:567-573.

 

6. Shyu, K.G..; Chen, J.J.; Shih, N.L.; Chang, H.; Wang, D.L.; Lien, W.P.; Liew, C.C.
(1995) “Angiotensinogen gene expression is induced by cyclic mechanical stretch in cultured rat cardiomyocytes.” Biochem. Biophys. Res. Commun. 211:241-248.

 

7. Huang, W.Y.; Chen, J.J.; Shih, N.L.; Liew, C.C.
(1997) “Multiple muscle-specific regulatory elements are associated with a Dnase I hypersensitive site of the cardiac beta-myosin heavy chain gene.” Biochem. J. 327:507-512.

 

8. Cheng, T.H.; Shih, N.L.; Chen, S.Y.; Wang, D.L.; Chen, J.J.
(1999) ”Reactive oxygen species modulate endothelin-1-induced c-fos gene expression in cardiomyocytes.” Cardiovasc. Res. 41:654-662.

 

9. Shih, N.L.; Cheng, T.H.; Loh, S.H.; Cheng, P.Y.; Wang, D.L.; Chen, Y.S.; Liu, S.H.; Liew, C.C.; Chen, J.J.
(2001) “Reactive oxygen species modulate angiotensin II-induced beta-myosin heavy chain gene expression via Ras/Raf/extracellular signal-regulated kinase pathway inneonatal rat cardiomyocytes.” Biochem. Biophys. Res. Commun. 283:143-148.

 

10. Cheng, T.H.; Shih, N.L.; Chen, S.Y.; Loh, S.H.; Cheng, P.Y.; Tsai, C.S.; Liu, S.H.; Wang, D.L.; Chen, J.J.
(2001) “Reactive oxygen species mediate cyclic strain-induced endothelin-1 gene expression via Ras/Raf/extracellular signal-regulated kinase pathway in endothelial cells.” J. Mol. Cell. Cardiol. 33:1805-1814.

 

11. Chen, J.J.; Shih, N.L.
(2002) “Myth of Linzhi, the mushroom of immortality: A potential anti-tumor/anti-aging agent in humans.” Acta Cardiol. Sinica. 18:113-114.

 

12. Cheng, C.M.; Hong, H.J.; Liu, J.C.; Shih, N.L.; Juan, S. H.; Loh, S.H.; Chan, P., Chen, J. J.; Cheng, T.H.
(2003) “Crucial role of extracellular signal-regulated kinase pathway in reactive oxygen species-mediated endothelin-1 gene expression induced by endothelin-1 in rat cardiac fibroblasts.” Mol Pharmacol. 63(5): 1002-11

 

13. Cheng, T.H.; Cheng, P.Y.; Shih, N.L.; Chen, I.B.; Wang, D.L.; Chen, J.J.
(2003) “Involvement of reactive oxygen species in angiotensin II-induced endothelin-I gene expression in rat cardiac fibroblast. “ J. Am. Coll. Cardiol. 42(10): 1845-54, 2003.

 

14. Chen, J.J.; Shih, N.L.
(2003) “Carvedilol : An anti-inflammatory and anti-atherogenic-blocker.” Acta Cardiol. Sinica. 19: 93-94.

 

15. Liu, J.C.; Cheng, T.H.; Lee, H.M.; Lee, W.S.; Shih, N.L.; Chen, Y.L., Chen, J.J.; Chan, P.
(2004) “Inhibitory effect of trilinolein on angiotensin II-induced cardiomyocyte hypertrophy.” Eur. J. Pharmacol. 484(1): 1-8.

 

16. Cheng, T.H.; Liu, J.C.; Lin, H.; Shih, N.L.; Chen, Y.L.; Huang, M.T.; Chan, P.; Cheng, CF.; Chen, J.J.
(2004) “Inhibitory effect of resveratrol on angiotensin II-induced cardiomyocyte hypertrophy.” Naunyn Schmiedebergs Arch Pharmacol. 369(2): 239-44.

 

17. Liu, J.C.; Chan, P.; Chen, J.J.; Lee, H.M.; Lee, W.S.; Shih, N.L.; Chen, Y.L.; Hong, H.J.; Cheng, T.H.
(2004) “The inhibitory effect of trilinolein on norepinephrine-induced beta-myosin heavy chain promoter activity, reactive oxygen species generation, and extracellular signal-regulated kinase phosphorylation in neonatal rat cardiomyocytes.” J. Biomed. Sci. 11(1): 11-8.

 

18. Cheng, T.H.; Shih, N.L.; Chen, C.H.; Lin, H.; Liu, J.C.; Chao, H.H.; Liou, J.Y.; Chen, Y.L.; Tsai, H.W.; Chen, Y.S.; Cheng, C.F.; Chen, J.J.
(2005) “Role of mitogen-activated protein kinase pathway in reactive oxygen species-mediated endothelin-I-induced β-myosin heavy chain gene expression and cardiac hypertrophy in neonatal rat cardiomyocytes.” J. Biomed. Sci. 12:123-133. (*equal contribution)

 

19. Cheng, T.H.; Shih, N.L.; Chen, S.Y.; Lin, J.W.; Chen, Y.L.; Chen, C.H.;? Lin, H.; Cheng, C.F.; Chiu, W.T.; and Wang, D.L.; Chen, J.J.
(2005). “Nitric Oxide Inhibits Endothelin-1- Induced Cardiomyocyte? Hypertrophy through cGMP- mediated Suppression of Extracellular- Signal Regulated Kinase Phosphorylation.” Molecular Pharmacology. 68:1183- 1192.

 

20. Chen C.H.; Cheng T.H.; Lin H.; Shih, N.L.; Chen Y.L.; Chen Y.S.; Cheng C.F.; Liang W.S.; Meng T.C.; Chiu W.T.; Chen J.J.
( 2006) “Reactive oxygen species generation is involved in epidermal growth factor receptor transactivation through the transient oxidization of Src homology 2-containing tyrosine phosphatase in endothelin-1 signaling pathway in rat cardiac fibroblasts.” Mol Pharmacol. 69(4):1347-1355.

 

21. Cheng P.Y.; Lee Y.M.; Shih, N.L.; Chen Y.C.; Yen M.H.
(2006) “Heme oxygenase-1 contributes to the cytoprotection of alpha-lipoic acid via activation of p44/42 mitogen-activated protein kinase in vascular smooth muscle cells.” Free Radic Biol Med. 40(8):1313-1322.

 

22. Cheng T.H.; Chen J.J.; Shih, N.L.; Lin J.W.; Liu J.C.; Chen Y.L.; Chen C.H.; Chen J.J.
(2009) “Mechanical stretch induces endothelial nitric oxide synthase gene expression in neonatal rat cardiomyocytes.”
Clin Exp Pharmacol Physiol. 2009 May;36(5-6):559-66.

 

23. Huang, Y.-S., Chang, C.-W., Chen, Y.-M., Lee, Y.-H., Chen, M.-C., Shih, N.-L.
(2010) “Investigating expression profiles of VEGF-Flk, and Angpt1 during development of gas glands in Japanese eel (Anguilla japonica) Comp.”
Biochem. Physiol., 155A:350-360. (IF=2.235;Rank=186/290,Biochemistry & Molecular Biology)

 

24.Tanshinone IIA Induces Heme Oxygenase 1 Expression and Inhibits Cyclic Strain-Induced Interleukin 8 Expression in Vascular Endothelial Cells.Zhuang S, Cheng TH, Shih NL, Liu JC, Chen JJ, Hong HJ, Chan P.Am J Chin Med.

2016 Apr;44(2):377-88.

 

25.Nicorandil Inhibits Cyclic Strain-Induced Interleukin-8 Expression in Human Umbilical Vein Endothelial Cells.
Chao HH, Hong HJ, Cheng TH, Shih NL, Loh SH, Liu JC, Chen JJ, Sung LC.Pharmacology.

2016;98(1-2):42-50.

 

26.Sinugyrosanolide A, an unprecedented C-4 norcembranoid, from the Formosan soft coral Sinularia gyrosa.
Cheng SY, Shih NL, Chuang CT, Chiou SF, Yang CN, Wang SK, Duh CY.Bioorg Med Chem Lett.

2014 Mar 15;24(6):1562-4.

 

27. Kelsoenethiol and dikelsoenyl ether, two unique kelsoane-type sesquiterpenes, from the Formosan soft coral Nephthea erecta.
Cheng SY, Shih NL, Hou KY, Ger MJ, Yang CN, Wang SK, Duh CY.Bioorg Med Chem Lett.

2014 Jan 15;24(2):473-5.