姓名:

黃永森 Yung-Sen Huang

職稱:

副教授兼任通識中心教學發展組組長

學歷:

國立海洋大學 水產養殖學系 學士
國立海洋大學 水產養殖研究所 碩士

法國巴黎第六大學 生殖生理學部 博士

經歷:

財團法人生物技術開發中心 ( 細胞生物及免疫學組 )
一般文書行政 ( 某軍事單位 )
台灣省水產試驗所 台西分所 助理
法國 Royan, CAREL 語文中心 修習法語
法國國家科學研究中心 Fessard 神經科學研究所 (Institut Alfred Fessard,UPR2212,CNRS,Gif-sur-Yvette France)
法國巴黎自然歷史博物館 (Museum National d'Histoire Naturelle, Paris France)一般及比較生理學實驗室 (Laboratoire de la Physiologie Generale et Comparee, UMR 8572 CNRS, Paris France)

行政院農業委員會水產試驗所 生物系 特聘研究員
行政院農業委員會水產試驗所 生物系 助理研究員
台北醫學大學 兼任助理教授
國立海洋生物博物館 企劃研究組 助理研究員
國立高雄大學 生命科學系 助理教授
國立高雄海洋科技大學 兼任助理教授

辦公室: 理學院大樓 502 室 
實驗室:

內分泌與血管內皮細胞生理實驗室(理學院大樓 502-1 室)

聯絡電話:
886-7-5919451,校內分機 7451
個人網站: www.pimpeneaucity.blogspot.com
E-Mail:

yshuang@nuk.edu.tw

學術專長:

內分泌學
分子內分泌學
生理學
生殖生理學

研究方向:

1. 放射性免疫分析 (RIA)
2. 酵素免疫分析 (ELISA)
3. 免疫沉澱分析 (Oudin test)
4. 核酸 (DNA, RNA) 操作
5. 基因選殖 (gene cloning)
6. 蛋白質純化 (Columns, utracentrifuge, HPLC)
7. 電泳操作 (RNA, proteins)
8. 單 , 多源抗體誘發 (monocolonial, polycolonial antibodies)
9. 動物細胞組織培養 (animal cell and tissue cultures)
10. 小動物外科手術
11. 個人電腦運用
研究成果主要以鰻魚(Anguilla spp.)作為模式生物,研究類固醇與代謝相關激素對動物生理之影響,主要研究成果包括:
1. 證明類胰島素成長因子(insulin-like growth factors, IGFs)可刺激 LH 的生成,而成長激素(growth hormone)無任何顯著作用,IGF 的生成主要由成長激素所調控,另外也受營養因子及代謝相關激素所影響,證明成長和生殖之間具化學訊息的存在(Huang et al., 1998; 1999)。

2.魚類的 LH 對類固醇激素之反應,與高等哺乳動物之間有其演化之保守性與歧異性。在高等哺乳動物中,雄性激素(testosterone)、可體素(cortisol)對 FSH 生成具專一性的刺激效果。在歐洲鰻中,我們發現 LH 可被 testosteronecortisol 刺激(Huang et al., 1999);進一步的並證明 testosteronecortisol 兩者不論在活體或離體條件下對 LH 的生成有加成的作用(Huang et al., 2001)。由活體與離體所得之相同結果顯示:鰻魚 LH 生成調控和哺乳動物 FSH 的生成調控具相似性。

3.以高屏溪的野生鰻魚為研究族群,調查台灣野生鰻魚之生理生態,建立臺灣野生鰻魚銀化變態過程的外觀型態與內在內分泌變遷資料(Han et al., 2000, 2003a,b)。

4.發現魚類大動脈血管內皮細胞(endothelial cells)之型態受類固醇激素影響(黃, 2002)。報告指出魚油所富含之 EPAeicospentaenoic acid),DHAdocosahexaenoic acid)可預防與舒緩人類之心血管疾病之發生及惡化,但未曾有報告研究類固醇激素對魚類血管系統之影響。選殖出鰻魚血管內皮細胞成長因子(Vascular endothelial growth factor, VEGF)、雌性素激素受體(E2 receptor)等基因片斷並發展為探針做為研究工具。

開授課程:

(大學部) 內分泌學生物統計學分子生物學中國傳統醫藥導論
(研究所) 環境荷爾蒙與內分泌專題研究

研究計劃:

1.計劃名稱:台灣海域珊瑚多樣性資源的現況評估與保育研究
計劃單位:國科會
執行期間:92.12.01~93.12.31
參與工作:主持人


2.計劃名稱:魚類血管組織在激素刺激下血管內皮成長因子之調控與表現
計劃單位:國科會
計劃編號:NSC 93-2313-B-291-005
執行期間:93.08.01~94.07.31
參與工作:主持人


3.計劃名稱:日本鰻魚成熟過程中血管生成之調控與應用(3/3) 
計劃單位:國科會
計劃編號:NSC 96-2313-B-390-002-MY3
執行期間:96.08.01~99.07.31
參與工作:共同主持人

 

著作專書:

 

1. Gwo, J.-C., Chang, C.-F., and Huang, Y.-S*

(2013) "PTEN on angiogenesis and ovarian development: experimental data from a primitive vertebrate, Anguilla japonica."In: Xu Ke (ed) PTEN: Structure, Mechanisms-of-Action, Role in Cell Signaling and Regulation. Nova Science Publishers, Inc., USA . (ISBN:978-1-62808-049-0)

期刊論文:

1. Chang, C.F.; Lee, F.Y.; Huang, Y.S.
(1993) "Purification and characetrization of vitellin from the mature ovaries of prawn, Penaeus monodon." Comp. Biochem. Physiol. 105B:409-414.

 

2. Chang, C.F.; Lee F-Y.; Huang, Y.S.; Hong, T.S.
(1994) "Purification and characterization of the female-specific protein (vitellogenin) in the mature female hemolymph of prawn, Penaeus monodon." Invert. Reprod. Dev. 25:185-192.

 

3. Huang, Y.S.; Schmitz, M.; Le Belle, N.; Chang, C.F.; Querat, B.; Dufour, S.
(1997) "Androgens stimulates gonadotropin-II b subunit in eel pituitary cells in vitro." Mol. Cell. Endocrinol. 131:157-166.

 

4. Huang, Y.S.; Le Belle, N.; Schmitz, M.; Chang, C.F.; Vernier P.; Querat, B.; Dufour, S.
(1998) “In vivo and in vitro effects of sex steroids on gonadotropin (GtH-II) synthesis in the female European eel.” Trends Comp. Endocrinol. Neurobiol., Ann. New York Acad. Sci. 839:348-350.

 

5. Huang, Y.S.; Rousseau, K.; Le Belle, N.; Vidal, B.; Burzawa-Gerard, E.; Marchelidon, J.; Dufour, S.
(1998) "Insulin-like growth factor I (IGF-I) stimulates gonadotropin production from eel pituitary cells: a possible metabolic signal for induction of puberty." J. Endocrinol. 159:43-52.

 

6. Huang Y.S.; Rousseau, K.; Sbaihi M.; Le Belle, N.; Schmitz, M.; Dufour, S.
(1999) "Cortisol selectively stimulates pituitary gonadotropin (GtH-II) b subunit, in a primitive teleost, Anguilla anguilla." Endocrinology 140:1228-1235.

 

7. Huang, Y.S.; Rousseau, K.; Le Belle, N.; Vidal, B.; Burzawa-Gerard, E.; Marchelidon, J.; Dufour S.
(1999) "Opposite effects of insulin-like growth factors (IGFs) on gonadotropin (GtH-II) and growth hormone (GH) production by primary culture of European eel ( Anguilla anguilla ) pituitary cells." Aquaculture 177:73-83.

 

8. Rousseau K.; Huang Y.S.; Le Belle, N.; Vidal B.; Marchelidon, J.; Epelbaum, J.; Dufour S.
(1998) "Long-term growth hormone release by serum-free primary culture of pituitary cells from European eel ( Anguilla anguilla ): inhibitory effects of somatostatin and insulin-like growth factors." Neuroendocrinology 67:301-309.

 

9. Chartrel, N.; Collin, F.; Huang, Y.S.; Montero, M.; Tonon, M. C.; Goos, H. J. Th.; Dufour, S.; Vaudry, H.
(1998) "Characterization and localization of two forms of gonadotropin-releasing hormone (GnRH) in the spinal cord of the frog Rana ridibunda." Cell Tissue Res. 293:235-243.

 

10. Han, Y.S.; Tzeng, W.N.; Huang, Y.S.; Liao, I.C.
(2000) "The silvering of Japanese eel Anguilla japonica: season, age size and fat." J. Taiwan Fish. Res. 8:37-45.

 

11. Jeng, S.R.; Chen, G.R.; Lai, J.Y.; Huang, Y.S.; Dufour, S.; Chang, C.F.
(2001) "Sex steroids and pituitary extract increase gonadotropin II (GtH-II) content in the pituitary of aquacultured Japanese eel, Anguilla japonica." J. Taiwan Fish. Res. 9:97-108.

 

12. Han, Y.S.; Tzeng, W.N.; Huang, Y.S.
(2001) "Silvering in the eel: Changes in morphology, body fat content, and gonadal development." J. Taiwan Fish. Res. 9:119-128

 

13. Jeng, S.R.; Chen, G.R.; Lai, J.Y.; Huang, Y.S.; Dufour, S.; Chang C.F.
(2002) 'Regulation of pituitary gonadotropin II and growth hormone content in the hormone-treated Japanese eels, Anguilla japonica." Aquaculture, 209:319-332.

 

14. Han, Y.S.; Liao, I.C.; Huang, Y.S.; He, J.T.; Chang, C.W.; Tzeng, W.N.
(2003) "Synchronous changes of morphology and gonadal development of silvering Japanese eel, Anguilla japonica." Aquaculture, 219:783-796.

 

15. Han, Y.S.; Liao, I.C.; Huang, Y.S.; Tzeng, W.N.; Yu, J.Y.L.
(2003) "Profiles of PGH-α, GTH I-β, and GTH II-β mRNA transcript levels at different ovarian stages in the wild female Japanese eel Anguilla japonica."
Gen. Comp. Endocrinol., 133:8-16.

 

16. Han, Y.S.; Liao, I.C.; Tzeng, W.N.; Huang Y.S.; Yu, J.Y.L.
(2003) "Serum estradiol-17 b and testosterone levels during silvering in wild Japanese eel, Anguilla japonica." Comp. Biochem. Physiol., 136B:913-920.

 

17. Huang, Y.S.; Wang, L.H.; Tsai, C.L.
(2004) "Photoperiod affects the development of central neurotransmitter system of tilapia, Oreochromis mossambicus." Neurosci. Letters, 355(3):201-204.

 

18. Huang, Y.S.; Huang, W.L.; Lin, W.F.; Chen, M.C.; Jeng, S.R.
(2006)  "An endothelial-cell-enriched primary culture system to study vascular endothelial growth factor (VEGF A) expression in a teleostean fish, Japanese eel (Anguilla japonica)." Comparative Biochemistry and Physiology,145A:33-46. (SCI)

 

19. Chen, Y.F.; Jeng, S.R.; Chen, M.C.; Gwo, J.C.; Huang, Y.S.
(2008) "Correlation Between PTEN and VEGF Expressions During the Development of Gas Gland in Japanese Eel (Anguilla japonica) Stimulated by Exogenous Hypophyseal Factors." The Open Zoology Journal, 1:1-10.

 

20. Hong, M.C.; Huang, Y.S.; Song,P.C.; Lin, W.W.; Fang, L.S.; Chen, M.C.

(2009) "Cloning and characterization of ApRab4, a recycling Rab protein of Aiptasia pulchella, and its implication in the symbiosome biogenesis." Marine Biotechnology 11 (6): 771-785.

 

21. Hong, M.C.; Huang,Y.S.; Lin, W.W.; Fang, L.S.; Chen, M.C.

(2009) "ApRab3, a biosynthetic Rab protein, accumulates on the maturing phagosomes and symbiosomes in the tropical sea anemone, Aiptasia pulchella." Comp. Biochem. Physiol. 152B: 249-259.

 

22.Huang,Y.S.; Chang, C.W.; Chen,Y.M.; Lee, ?Y.H.; Chen, M.C.; Shih, N.L.

(2010) "Investigating expression profiles of VEGF-Flk, and Angpt1 during development of gas glands in Japanese eel (Anguilla japonica)" Comp. Biochem. Physiol., 155A:350-360.

 

23. J. Fernandez-Tajes, A. Longa, J. Garcia-Gil, Chiu, Y.-W. and Huang, Y.-S., J. Mendez and R.-S. Lee

(2011) "Alternative PCR–RFLP methods for mussel Mytilus species identification." European Food Research and Technology. 233:791-796.(IF=1.585;Rank=25/115,Food Science).

 

24. Huang, Y.-S.*, Y.-M. Chen, P.-C. Liao, Y.-H. Lee, J.-C. Gwo, M.-C. Chen, C.-F. Chang

(2012) Testosterone improves the transition of primary oocytes in artificial maturation eels (Anguilla japonica) by altering ovarian PTEN expression Fish Physiology and Biochemistry, doi 10.1007/s10695-011-9560-6. (IF=1.607;Rank=25/115,Zoology)

 

25. Lin,C.-W.,Chen,G..-R., Wu,F.-C., Liu,F.-G.. and Huang,Y.-S.*

(2012) "Correlation between lipid content and nuclear receptor (PPARs, RXRalpha) as well as EDF-1 gene expression levels in muscle tissues of Anguilla japonica during growth." The Journal of the Fisheries Society of Taiwan. (in press).

 

26. Huang Y.-S., Gwo J.-C., Chen, Y.-F., Chang C.-F.

(2014) Correlation between PTEN expression levels and ovarian development in the induced Japanese eel. 7th Intercongress symposium of the Asia and Oceania Society for Comparative Endocrinology, Keelung Taiwan, Mar. 18-23. P81.

研討會論文:

1. Dufour, S.; Huang, Y.S. ; Rousseau, K.; Sbaihi, M.; Le Belle, N.; Vidal, B.; Marchelidon, J.; Querat, B.; Burzawa-Gerard, E.; Chang, C.F.; Schmitz, M.;
(2000) "Puberty in teleosts: new insights into the role of peripheral signals in the stimulation on pituitary gonadotropins." Proceedings of thr 6 th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp455-461.

 

2. Liao, I.C.; Huang, Y.S.
(2000) "Methodological approach used for the domestication of potential candidates for aquaculture." Seminar on Mediterranean Marine Aquaculture Finfish Species Diversification, Zaragoza-Spain, May 24-27, 1999. pp97-108.

 

3. Huang Y.S.; Sbaihi, M.; Le Belle, N.; Dufour, S.
(2001) "Combination of testosterone with cortisol stimulates the silverimg of female European eel ( Anguilla anguilla L.)" The 4 th Congress of the Asia and Oceania Society for Comparative Endocrinology, Taipei-Taiwan, May 14-18, 2000. Recent Advances in Comparative Endocrinology 2001. pp.215-222.

 

學術會議摘要 :

1. Huang Y.S., Le Belle, N.; Schmitz, M.; Chang, C.F.; Vernier, P.; Querat, B.; Dufour, S.
(1996) "In vitro and in vivo effects of sex steroids on gonadotropin (GtH-II) synthesis in the female European eel." The XVII Conference of European Comparative Endocrinologist: from molecular to integrative biology (September 10th-14th 1996, Rouen-France). Annales d'Endocrinologie, vol. 57, Suppl. No.4, p72.

 

2. Huang Y.S.; Dufour, S.
(1997) "Controle de la production d’hormone gonadotrope hypophysaire de type-II (GtH-II) chez un poisson teleosteen, l’anguille ( Anguilla anguilla L.)." troisieme Congres des Etudiants de 3eme cycle au Museum Natiomal d’Histoire Naturelle (11 et 12 juin 1997, Paris), p. 41.

 

3. Huang, Y.S.; Rousseau, K.; Le Belle, N.; Vidal, B.; Burzawa-Gerard, E.; Marchelidon, J.; Dufour, S.
(1997) "Opposite effects of insulin-like growth factors (IGFs) on gonadotropin (GtH-II) and growth hormone (GH) production by primary culture of European eel ( Anguilla anguilla ) pituitary cells." 3 rd International Symposium on Research for Aquaculture: Fundamental and Applied Aspects ( September 24 th-27 th 1997, Barcelona-Spain), p. 20.

 

4. Sbaihi, M.; Marchelidon, J.; Huang, Y.S.; Vidal B.; Querat, B.; Mayer, I.; Meunier, F.; Fouchereau-Peron, M.; Dufour, S.
(1997) "Conger and anguilla: a comparative approach for the study of puberty." 3 rd International Symposium on Research for Aquaculture: Fundamental and Applied Aspects ( September 24 th-27 th 1997, Barcelona-Spain), p. 36.

 

5. Marchelidon, J.; Adam, G.; Vidal, B.; Huang, Y.S.; Elie, P.; Dufour, S.
(1997) "Sex related growth and silvering of the eel ( Anguilla anguilla L.): implication of gonadotropic and somatotropic hormones." 3 rd International Symposium on Research for Aquaculture: Fundamental and Applied Aspects ( September 24 th-27 th 1997, Barcelona-Spain), p. 107.

 

6. Huang, Y.S.; Rousseau, K.; Marchelidon, J.; Dufour, S.
(1997) "Growth and puberty in fish: investigation on neuroendocrine interactions between somatotropic and gonadotropic axes in the European eel, Anguilla anguilla."
International Symposium on Fish Reproduction and Growth Physiology, Keelung, Taiwan, November 10-15, 1997.

 

7. Dufour, S.; Huang, Y.S.
(1998) "Role of growth and metabolic fators in the regulation of gonadotropin production in the eel, Anguilla anguilla." Conference on Reproduction and Aquaculture, National Taiwan Ocean University, Keelung, Taiwan, May 1, 1998.

 

8. Liao, I.C.; Huang, Y.S.
(1999) "Methodological approach used for the domestication of potential candidates for aquaculture." Seminar on Mediterranean Marine Aquaculture Finfish Species Diversification, Zaragoza-Spain, May 24-27, 1999.

 

9. Huang, Y.S.; Sbaihi, M.; Le Belle, N.; Dufour, S.
(2000) "Combination of testosterone with cortisol stimulates the silverimg of female European eel ( Anguilla anguilla L.)" The 4 th Congress of the Asia and Oceania Society for Comparative Endocrinology, Taipei-Taiwan, May 14-18, 2000. p10.

 

10. S baihi, M.; Huang, Y.S.; Le Belle, N.; Vidal, B.; Burzawa-Gerard, E.; Meunier, F.; Dufour, S.
(2000) "Effects of sexual steroids and cortisol on the reproductive axis and the mobilization of the skeleton and the energetic stores in the eel Anguilla anguilla." Conference of European Comparative Endocrinologists Year 2000, University of Algarve, Centre of Marine Sciences, Faro, Portugal, Sept. 5-9 th, 2000.

 

11. Dufour, S. ; Rousseau, K.; Sbaihi, M.; Schmitz, M.; Huang, Y.S.; Chang, C.F.; Chow, B.K.; Montero, M.; Vaudry, H.; Le Belle, N.; Vidal, B.; Marchelidon, J.
(2000) "Multiple control of growth hormone production in the European eel. " The 3 rd East Asian Symposium on Eel Research – Sustainability of Resources and Aquaculture of Eels, Keelung-Taiwan, Nov. 16-18, 2000. pp25-27.

 

12. Han, Y.S.; Huang, Y.S.; Liao, I.C.; Tzeng, W. N.
(2001) "Age and energy store of the silvering Japanese eel in the Kaoping river of southwestern Taiwan." Asian Fisheries Society, 6 th Asian Fisheries Forum, Book of Abstracts, Kaohsiung, Taiwan, Nov. 25-30, 2001. p89.

 

13. Huang, Y.S.; Lan, H.L.; Wang, W.C.; Liao, I.C.
(2001) "Body composition changes during long-term starvation of the Japanese eel Anguilla japonica at the glass eel stage." Asian Fisheries Society, 6 th Asian Fisheries Forum, Book of Abstracts, Kaohsiung, Taiwan, Nov. 25-30, 2001. p106.

 

14. Huang, Y.S.
(2002) "Preliminary investigation for effects of steroid hormones on the morphological modifications of eel (anguilla Japonica) aorta-inner-wall cells." 台灣省水產學會 91 年度學術論文發表會 . Taipei, Taiwan, Dec. 15-16, 2002. pE-17.

 

15. Huang, Y.S.; Cheng, T.C.
(2004) "Clonning and characterization of vascular endothelial growth factor (VEGF) gene from Japanese eel, Anguilla japonica." The 5 th Congress of the Asia and Oceania Society for Comparative Endocrinology, Nara-Japan, March 26-30, 2004. px

 

16. Tai, H.C.; Chen, Y.F.; Lin, C.H.; Chen, G.R.; Huang, Y.S.
(2006) "Yin (PTEN) and Yang (VEGF) control ovarian developmentin the Japanese eel by exogenous gonadotropic treatments Ecophysiology in Marine Organism." National Taiwan Ocean University Keelung, October 3-4, 2006.

 

17. 陳逸帆, 戴豪志, 林芷歆, 鄭絢如, 黃永森
(2006) "PTEN, but not VEGF, is the main factor to effect the development of ovary and gas gland (rete mirabile) in Japanese eel (Anguilla japonica) stimulated by catfish pituitary extracts." 2006 Symposium on Recent Advances in Biotecnology, NUK. Nov. 2-3.

 

18. 陳雅玫, 韓玉山, 黃永森
(2007) "高屏溪日本鰻氣腺組織血管新生相關基因之調查" 台灣省水產學會96年度學術論文發表會. Koushiung, Taiwan, Dec. 15-16, 2007. pE-129.

 

19. 馮仕成, 陳逸帆, 陳雅玫, 黃永森
(2007) "不同過氧化物增殖因子活性受體配體增強物對鰻魚紅體細胞血管新生相關基因表現的影響" 台灣省水產學會96年度學術論文發表會. Koushiung, Taiwan, Dec. 15-16, 2007. pE-131.

 

20. 陳逸帆, 戴豪志, 林芷歆, 鄭絢如, 黃永森

(2007)" Role of PTEN or VEGF on the postnatal tissue development in a lower vertebrate (Japanese eel, Anguilla japonica)." The 15th Symposium on Recent Advances in Cellular and Molecular Biology. Kenting, Taiwan. Feb. 1-3, 2007. p248.

 

21.陳逸帆, 陳雅玫, 黃永森

(2008) "鰻魚(Anguilla japonica) PTEN基因選殖與其在卵巢、紅體組織中表現量之變化" ,第二屆海峽兩岸『魚類生理與養殖』學術研討會. 中國武漢, Oct. 13-15, 2008. p12-13.

 

22. Chang, C.W.;Chen, Y.M.; Lee, Y.H.; Huang, Y.S.

(2009) "Does Testosterone benefit the artificial ovarian growth in the eel (Anguilla japonica) by altering the expression levels of VEGF, FLK, and Eph receptor? " 16th International Congress of Comparative Endocrinology (ICCE2009), Hong Kong, Jun. 22-26, 2009. p117.

 

23.張宸瑋, 陳雅玫, 李彥宏, 黃永森

(2009) "添加睪固酮改善鰻魚(Anguilla japonica)卵巢人工催熟發育之探討-是否改變血管新生基因表現量 ?" 第三屆海峽兩岸『魚類生理與養殖』研討會. 台灣基隆, Oct. 19-21, 2009. p34-35.

 

24. 林家緯, 陳冠如, 李彥宏, 劉富光, 黃永森

(2009) "鰻魚體脂肪含量變化與其能量代謝基因表現之研究" 台灣省水產學會98年度學術論文發表會. Kaohsiung, Taiwan, Dec. 19-20, 2009. p-317

 

25. 黃永森*

(2015) PTEN or Cilostazol on artificial-induced ovarian development in the Japanese eel, Anguilla japonica. 台灣生物科技產學新知 – 水產動物生理。台灣 台南市. Apr. 29, 2015. P10.

 

26. 黃永森*

(2015) 大海撈針 – 尋找鰻魚產卵場。海洋科學與應用學術研討會。海軍軍官學校. 台灣 高雄左營. Oct. 06, 2015. P158.

特殊榮譽:

博士論文被巴黎大學評為 ‘特優’ (評比分佈位於10%以內)
國科會八十八學年度甲種研究獎勵 (動物學門)
國科會八十九學年度乙種研究獎勵 (漁業學門)
名列美國 Marquis Who's Who「世界名人錄」2009/2010
名列2000 Outstanding Intellectuals of the 21st Century (International Biographical Centre, Cambridge, England) 2009/2010