Curriculum Vitae

Name:Fang-Sheng Wu

Office Address: Department of Biology                          
Tel:(804) 828-0755 Box 842012, 1000 W. Cary ST.                                       
Virginia Commonwealth University               
Fax:(804) 828-0503  Richmond, Va.  23284-2012                      
E-mail address:FWU@VCU.EDU                                   

Employment
1988-         Associate Professor, Department of Biology; Virginia Commonwealth University,
      Richmond, VA.  Tenured,1991. 
1981-1988  Senior Biologist, Plant Biotechnology Division, Sandoz Crop Protection, Sandoz Ltd.
      (now Norvatis), Palo Alto, California.        
1979-1981  Research Assistant Professor, Department of Biochemistry, Upstate Medical Center,
      State University of New York, Syracuse, N.Y.
1977-1979  Postdoctoral Fellow, Biochemistry and Biophysics Section, University of Connecticut,
      Storrs, Connecticut.

Education:
Ph.D.    Dept. of Botany and Plant Pathology, Michigan State University. 
    Areas of study: Cell and Molecular Biology/Plant Pathology. 1977
M.S.     Biological Sciences Institute, National Taiwan Normal University. 
    Areas of study: Microbiology   
B.S.     Biology Department, National Taiwan Normal  University.
    Major: Biology    Minor:Botany 

Courses Taught at Virginia Commonwealth University:
Bio 399E Recombinant DNA, Honors Program
Bio 310  Genetics, 3 credits
Bio 310z Genetics Laboratory, 2 credits
Bio 492  Independent Study, credits varied
Bio 540  Fundamental Molecular Genetics
Bio 502/Mic 502 Microbial Biotechnology, 3 credits
Bio 580 Eukaryotic Biotechnology, 3 credits.  
Bio 591 Special Topics in Genetic Engineering, 3 credits
Bio 690 Research Seminar, 1 credit
Bio 691 Special Topics in Plant Molecular Biology,3 credits

Major Advisor for the following graduate students (M.Sc. and Ph.D):
Cahoon, Bruce; Cordsman, Alex; Gargaliadi,Steve; Lee,Chih-Chung; Lesniewski, Matthew; Morehouse,Christophore; Peterson, Erica; Redmond, Latasha; Reyad, Ahmed Hassan
(University of Egypt– Egypt government sponsored Ph.D.);  Saliaah, Katrina
(US Air Force-sponsored Ph.D.); Shaaban, Enas (University of Egypt – Egypt government
sponsored Ph.D.); Shuleeta, Maria; Sun, Deqin; Tayler, Scott.  

Selected Professional Activities:
Visiting Professor: Academia Sinica. Three months at a time during summers, five times.
Visiting Scholar: Taiwan Seed Service, Taichung, Taiwan. Three months at a time during summers,
    three times.
Visiting Scholar: Taiwan Agricultural Research Institute.  May-August, 2010
Visiting Senior Scientist, Sungene Corporation, San Jose, California. Worked on a biofuel project
    in a summer.
Visiting Professor: Institute of Genetics, Chinese Academy of Science; Chinese Agricultural University,
    Beijing, China. One each in summer.
Panel member, Study section of Microbiological and Immunological Sciences Special Emphasis panel.
    National Institute of Health, USA.
Panel member, Nationwide Review Panel of Agricultural Biotechnology Research in Taiwan.  Council of
    Agriculture, R.O.C. Two weeks.1994.
Participant, workshop on Research Methods for Somatic Cell and Molecular Genetics
    (Supported by National Cancer Institute, USA) at Eleanor Roosevelt Institute, Denver,                
    Colorado. A workshop with intensive hands-on experiments. Two weeks.
Consultant, Biochemical Research Div., Philip Morris USA. On as-needed basis. 1996-2006.  
Advisory Panel member, Plant Tissue Culture Section,Programs in Science and Technology Coop,     
    and Programs in U.S.-Israel Cooperative Development Research, Office of the Science Advisor,
    U.S. Agency for International Development, Washington, D.C.
Reviewer for grant proposals submitted to Virginia Center for Innovative Technology, Institute of
    iotechnology, Virginia.   
Reviewer for grant proposals submitted to North Carolina Biotechnology Institute, North Carolina.
Advisory review panel member, SBIR program, Molecular Bioscience Division, National Science
    Foundation, Washington, D.C. 1988
Consultant for Educational Testing Service of USA for AP Biology. Three summers.
Reviewer for various journals.

Selected Publications (not inclusive):
1.  Jaye, M., F.S. Wu and J.M. Lucas-Lenard.  INHIBITION OF SYNTHESIS OF RIBOSOMAL PROTEINS
  AND OF RIBOSOME ASSEMBLY AFTER INFECTION OF L CELLS WITH VESICULAR STOMATITIS   
  VIRUS. Biochem. Biophys. Acta606, 1-12 (1980).
2.  Wu, F.S. and J.M. Lucas-Lenard.  INHIBITION OF RIBONUCLEIC ACID ACCUMULATION IN MOUSE
  L CELLS INFECTED WITH VESICULAR STOMATITIS VIRUS REQUIRES VIRAL RIBONUCLEIC ACID   
  TRANSCRIPTION. BIOCHEMISTRY 19, 804-810 (1980).
3.  Dikla Roufa, F.S. Wu and A.N. Martonosi.  THE EFFECT OF Ca2+ -IONOPHORES UPON THE
  SYNTHESIS OF PROTEINS IN CULTURED SKELETAL MUSCLE. Biochem. Biophys. Acta. 674,
  225-237 (1981).
4.  Wu, F.S., Y-C Park, D. Roufa and A.N. Martonosi. SELECTIVE STIMULATION OF THE SYNTHESIS
  OF AN 80,000 PROTEIN BY Ca2+ IONOPHORES. J. of Biol. Chem. 256, 5309-5312 (1981).
5.  Wu, F.S., G.R. Hooper, and H. Murakishi. CYTOPATHOLOGY OF SOYBEAN TISSUE CULTURE
  CELLS INFECTED WITH SOUTHERN BEAN MOSAIC VIRUS. In vitro 6, 525-530 (1982).
6.  Wu, F.S. and M. Wang.  EXTRACTION OF PROTEINS FOR SODIUM DODECYL SULFATE
  POLYACRYLAMIDE GEL ELECTROPHORESIS FROM PROTEASE-RICH PLANT TISSUES.
  Analy. Biochem. 139, 100-103 (1984).
7.  Wu, F.S. and Lynn Murry.  PROTEOLYTIC ACTIVITY IN ANTHER EXTRACTS OF FERTILE AND
  CYTOPLASMIC MALE STERILE PETUNIA. Plant Physiology. 79, 301-305 (1985).
8.   Wu, F.S. and Lynn Murry. CHANGES IN PROTEIN AND AMINO ACID CONTENT DURING ANTHER
  DEVELOPMENT IN FERTILE AND CYTOPLASMIC MALE STERILE PETUNIA.
  Theor. Appl. Genet. 71, 68-73 (1985).
9. Wu, F.S.  LOCALIZATION OF MITOCHONDRIA IN PLANT CELLS BY VITAL STAINING WITH
  RHODAMINE 123. Planta 171:346-357 (1987).
10. Trail, F., C. Richards and F.S. Wu.  GENETIC MANIPULATION IN BRASSICA.
  In: BIOTECHNOLOGY IN AGRICULTURE AND FORESTRY.  Edited by Y.P.S. Bajaj.  Pub.
  Springer-Verlag. Vol 9, pp197-216. (1989).
11. Wu, F.S. and Tsai, Y-Z.  EVACUOLATION AND ENUCLEATION OF MESOPHYLL PROTOPLASTS
  IN SELF-GENERATING PERCOLL GRADIENTS.  Plant, Cell and Environment 15:685-692  (1992).
12. Ha, Sam B., Wu, F-S., and Thornd, T.K. TRANSGENIC TALL FESCUE FESTUCA ARUNDINACEA
  SCHREB.) PLANTS REGENERATED FROM PROTOPLASTS. Plant Cell ReportS  11:601-604 (1992).
13. Wu, F.S., Cahoon, A.B., and Shulleeta.  DELIVERY OF PROTEINS AND DNA INTO INTACT PLANT
  CELLS.  In Vitro Cellular and Develo. Biol.30A:62 (1994).
14. Ha, Sam B., Penmetsa, R.V., and Wu, F.S. TRANSIENT EXPRESSION OF THE
  BETA-GLUCURONIDASE GENE DRIVEN BY DIFFERENT 5' REGULATORY SEQUENCES IN TALL
  FESCUE PROTOPLASTS.  J. of Turfgrass Management 1: 73-82. (1995). 
15. Wu, F.S., and Cahoon, A.B. PLASMOLYSIS FACILITATES THE ACCUMULATION OF PROTEIN AND
  DNA INTO EXTRA-PLASMALEMMA SPACES OF INTACT PLANT CELLS. Plant Science.
  104: 201-214 (1995)
16. Wu, F.S., and Feng, T-Y, Delivery of plasmid DNA into intact plant Cells by Electroporation of
  plasmolyzed cells. Plant Cell Reports 18:381-386 (1999).
17. Lee,H., Sun,D. Larner, J. M., and Wu, F.S. The tumor suppressor p53 can reduce stable transfection
  in the presence of irradiation.       J. Biomedical Science 6:285-292 (1999).
18. Hui, L, F-S Wu, D-W  Wang,, J. Xu.  Wheat Transformation by Electroporation with Ring Electrode.
  Acta Genetica Sinica 52:66-71 (2005).
19. Sun, Y-W., Liao, Y-J., Chang, Y-H., Chen, K-S and Wu,F-S.  Classification of Paphiopedilum Genus
  into Three Subgenera and Parental Identification of P. delenatii in Hybrids by ITS Molecular Marker.
  International Orchid Symposium (under the auspices of the International Society for Horticultural
  Science), 12-P-28, (2010).
20. Y. -W. Sun1, J. -J. Chen1, W. -N. Chang2, M. -J. Tseng2, and F.-S. Wu*. Irrigation with 5°C water
  and paclobutrazol promote strong   seedling growth in tomato (Solanum lycopersicon).
  
J. of Hort. and Biotech. 85: 305-311, (2010). 
21. Yi-Hsien Lina, Hsiang-EnHuang, Fang-Sheng Wu, Mang-JyeGerd, Pei-LuanLiaoa, Yen-Ru Chena,
  Kuo-ChingTzenge, Teng-Yung Feng.
  Plant ferredoxin-likeprotein(PFLP)outside chloroplast in Arabidopsis enhances disease resistance   
  against bacterial pathogens.  Plant Science 179: 450-458(2010).

 



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