Curriculum Vitae

Family name: De Keyser
Names:  Ellen Canisia
Address (office):  Caritasstraat 21, 9090 Melle
E-mail:   ellen.dekeyser@ilvo.vlaanderen.be
Tel: 00 32 9 272.29.43 (office)
Place of Birth: Gent
Nationality: Belgian

Education:
Secundary school (1989-1995)

Science-mathematics (8u mathematics)
Sint-Bavohumaniora – Gent
University (1995-2000)
Bio-engineer in cell and gene biotechnology
Gent University
Thesis: “Selection and characterisation of elite transgenic lines by means of anchor- -PCR”
Promoter: Prof. Dr. ir. Erik Van Bockstaele
Doctoral training (2001-2006)
Doctoral training in applied biological sciences
PhD (2010)
Submitted to the Faculty Board on September 22th 2010, defence scheduled on December 20th 2010.
Title: Integration of phenotype, genotype and gene expression to unravel flower colour biosynthesis and complex plant quality traits in azalea (Rhododendron simsii hybrids).

Professional experience:
17/07/2000-today:
Scientific attaché at ILVO-Plant Sciences Unit-Applied Genetics and Breeding

Publications:

Peer-reviewed
Scariot V, De Keyser E, Handa T and De Riek J (2007). Comparative study of the discriminating capacity and effectiveness in assessing genetic relationships of AFLP, STMS and EST markers in evergreen azaleas. Plant Breeding 126: 207-212.

Eeckhaut T, De Keyser E, Van Huylenbroeck J, De Riek J and Van Bockstaele E (2007). Application of embryo rescue after interspecific crosses in the genus Rhododendron. Plant Cell Tissue and Organ Culture 89: 29-35.

De Keyser E, De Riek J and Van Bockstaele E (2009). Discovery of species-wide EST-derived markers in Rhododendron by intron-flanking primer design. Molecular Breeding 23: 171-178.

De Keyser E, Shu QI, Van Bockstaele E and De Riek J (2010). Multipoint-likelihood maximization mapping on 4 segregating populations to achieve an integrated framework map for QTL analysis in pot azalea (Rhododendron simsii hybrids). BMC Molecular Biology 11:1.

Other publications

De Keyser E, De Riek J and Van Bockstaele E (2004). Optimisation of relative quantitative RT-PCR for expression analysis in azalea flower colour sports. Acta Horticulturae 651: 91-96.

De Keyser E, Scariot V and De Riek J (2007). Development of EST markers and evaluation of their use in evergreen azalea analysis. Acta Horticulturae 743: 107-113.

De Keyser E, De Riek J and Van Bockstaele E (2007). Gene expression profiling of key enzymes in azalea flower colour biosynthesis. Acta Horticulturae, 743: 115-120.

De Keyser E, Scariot V, Handa T and De Riek J (2008). EST markers in evergreen azalea: development and use for classifications compared to AFLP, microsatellite and morphological data. Acta Horticulturae: 763: 107-115.

De Keyser E, Heungens K, Pauwels E and De Riek J (2008). Development of supporting techniques for pot azalea (Rhododendron simsii hybrids) breeding focused on plant quality, disease resistance and enlargement of the assortment. Acta Horticulturae 766: 361-366.

De Keyser E, Lootens P, De Riek J and Van Bockstaele E (2009). Flower colour as a model in azalea for integration of phenotype, genotype and gene expression. Acta Horticulturae 836: 49-54.

Books

De Riek J, Scariot V, Eeckhaut T, De Keyser E, Kobayashi N and Handa T (2008). The potential of molecular analysis and interspecific hybridization for azalea phylogenetic research. In: Sharma AK and Sharma A (eds.). Plant Genome, Biodiversity and Evolution. Volume 1, part E: Phanerograms – Angiosperm. Science Publishers, Enfield, NH, USA pp 251-302.

De Keyser E, Scariot V, Kobayashi N, Handa T and De Riek J (2010). Azalea phylogeny reconstructed by means of molecular techniques. In: Jain SM and Ochatt SJ (eds.). Protocols for In Vitro Propagation of Ornamental Plants, Methods in Molecular Biology 589, Humana Press, Springer, New York, pp 349-364.

Eeckhaut T, Janssens K, De Keyser E and De Riek J (2010). Micropropagation in Rhododendron. In: Jain SM and Ochatt SJ (eds.). Protocols for In Vitro Propagation of Ornamental Plants, Methods in Molecular Biology 589, Humana Press, Springer, New York, pp 141-152.

Scientific activities:

Lectures on international conferences

De Keyser E, De Riek J and Van Bockstaele E (2003). Optimisation of relative quantitative RT-PCR for expression analysis in azalea flower colour sports. Eucarpia, 21st international symposium section ornamentals: Classical versus molecular breeding of ornamentals, 25-29 Augustus 2003, Freising-Weihenstephan, Germany (poster presentation).

De Keyser E, De Riek J and Van Bockstaele E (2003). The use of relative quantitative RT-PCR for expression analysis in azalea flower colour sports. FAB, 17th meeting, 17-18 September 2003, Gent, Belgium.

Uytterhaegen R, De Keyser E and De Riek J. Current objectives for the breeding and research in Belgian pot azaleas. Camelia e azalea: due preziosità botaniche dall’Oriente, 12-13 Mai 2006, Verbania, Italy.

De Keyser E, Scariot V and De Riek J. Development of EST markers and evaluation of their use in evergreen azalea analysis. Eucarpia, 22nd international symposium section ornamentals: Breeding for beauty, 11-15 September 2006, San Remo, Italy (poster presentation).

De Keyser E, De Riek J and Van Bockstaele E. Gene expression profiling of key enzymes in azalea flower colour biosynthesis. Eucarpia, 22nd international symposium section ornamentals: Breeding for beauty, 11-15 September 2006, San Remo, Italy (poster presentation).

De Keyser E, Desmet L, De Riek J and Van Bockstaele E. Development of a consistent protocol for qPCR analysis in azalea flowers.Benelux qPCR symposium, 6 October 2008, Gent, Belgium.

De Keyser E, Lootens P, De Riek J and Van Bockstaele E (2009). Flower colour as a model in azalea for integration of phenotype, genotype and gene expression. Eucarpia, 23rd international symposium section ornamentals: Colourful breeding and genetics. 31 August - 4 September 2009, Leiden, The Netherlands.

De Keyser E, Desmet L, Van Bockstaele E and De Riek J. Challenges of using qPCR for eQTL mapping. Advances in Genomics, 28-29 January 2010, Gent, Belgium.

International collaboration

Study to Japan with lectures on diverse universities. 12 Mai-9 June 2007 (FWO scolarship).
Partner in ForESTflowers: exchange program for senior researchers with China, Japan, France and Italy (EU –IRSES – Marie-Curie)

Education
Guest lecturer Avans Hogeschool (Breda, The Netherlands). Course ‘Quantitative PCR techniques’. 8-10 July 2009. Development of a reliable protocol for qPCR analysis in azalea flowers.




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